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BGC0002350Hybrid

bleomycin A2;bleomycin B2

Producing organism
Streptomyces mobaraensis NBRC 13819 = DSM 40847
Taxonomy
Bacteria›Actinomycetota›Actinomycetes›Kitasatosporales›Streptomycetaceae›Streptomyces
Biosynthetic class
NRPS (Type 1);PKS (Unknown);saccharide (None)
Total steps
19
DOI
MIBiG entry
View on MIBiG ↗
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Gene Cluster Map41 genes · 90,958 bp

CAL20 kb91.0 kb visible · 90,958 bp total
Biosynthetic (core)
Biosynthetic (additional)
Regulatory
Transport
Other
Unknown
Domains
KSKetosynthase (KS)
ATAcyltransferase (AT)
DHDehydratase (DH)
KRKetoreductase (KR)
ACPAcyl carrier protein (ACP)
ACPAcyl carrier (ACP)
CCondensation (C)
AAdenylation (A)
PCPPeptidyl carrier (PCP)
ACPAcyl carrier (ACP)
TEThioesterase (TE)
CyHeterocyclization (Cy)
MTMethyltransferase
CALCoA-ligase (CAL)
TauDTauD oxygenase

Pathway Graphsteps arranged in biosynthetic order

19 steps · 20 edges
Step 1M1
J7W19_00430
+L-Serine
Step 16
Start
+L-Gulose
Step 17
Start
+3-O-Carbamoyl-D-m…
Step 2M2
J7W19_00430
+L-Asparagine
Step 3M3
J7W19_00410
+L-Asparagine
Step 4M4
J7W19_00410
+L-Histidine
Step 5M5
J7W19_00415
+L-Alanine
Step 6M6
J7W19_00420
+Malonyl-CoA
Step 7
?
Step 8M7
J7W19_00425
+L-Threonine
Step 9M8
J7W19_00385
+beta-Alanine
Step 10M9
J7W19_00385
+L-Cysteine
Step 11M10
J7W19_00385,J7W19_00380
+L-Cysteine
Step 12
?
Step 13
?
Step 14
?
+terminal amine
Step 15
?
+terminal amine
Step 18
?
Step 19
?
Starting stepIntermediateFinal product│ModuleLoadingTENo module│PKS stepNRPS stepTailoring/otherScroll to pan · Wheel to zoom

Biosynthetic Reactions19 steps

StepProduct IDEnzymeModuleSubstrateProduct SMILESNotes
12350-1J7W19_00430M1
L-Serine
OC[C@]([H])(N)C(N)=O—
22350-2J7W19_00430M2
2350-1;L-Asparagine
from: 2350-1
O=C(N)C[C@](C(O)=O)([H])NC[C@]([H])(N)C(N)=O—
32350-3J7W19_00410M3
2350-2;L-Asparagine
from: 2350-2
O=C(N)C[C@](C1=NC(N)=C(C)C(C(O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
42350-4J7W19_00410M4
2350-3;L-Histidine
from: 2350-3
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@](C(C2=CNC=N2)([H])[H])([H])C(O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
52350-5J7W19_00415M5
2350-4;L-Alanine
from: 2350-4
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@](C(C2=CNC=N2)([H])[H])([H])C(N[C@@]([H])(C)C(O)=O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
62350-6J7W19_00420M6
2350-5;Malonyl-CoA
from: 2350-5
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@](C(C2=CNC=N2)([H])[H])([H])C(NC(C)([H])C([C@]([H])(C)C(O)=O)=O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
72350-7?—
2350-6
from: 2350-6
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@](C(C2=CNC=N2)([H])[H])([H])C(N[C@@](C)([H])[C@@]([C@]([H])(C)C(O)=O)([H])O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
82350-8J7W19_00425M7
2350-7;L-Threonine
from: 2350-7
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@](C(C2=CNC=N2)([H])[H])([H])C(N[C@@](C)([H])[C@@]([C@]([H])(C)C(N[C@@](C(O)=O)([H])[C@@](O)([H])C)=O)([H])O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
92350-9J7W19_00385M8
2350-8;beta-Alanine
from: 2350-8
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@](C(C2=CNC=N2)([H])[H])([H])C(N[C@@](C)([H])[C@@]([C@]([H])(C)C(N[C@@](C(NCCC(O)=O)=O)([H])[C@@](O)([H])C)=O)([H])O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
102350-10J7W19_00385M9
2350-9;L-Cysteine
from: 2350-9
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@](C(C2=CNC=N2)([H])[H])([H])C(N[C@@](C)([H])[C@@]([C@]([H])(C)C(N[C@@](C(NCCC3=NC(C(O)=O)=CS3)=O)([H])[C@@](O)([H])C)=O)([H])O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
112350-11J7W19_00385,J7W19_00380M10
2350-10;L-Cysteine
from: 2350-10
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@](C(C2=CNC=N2)([H])[H])([H])C(N[C@@](C)([H])[C@@]([C@]([H])(C)C(N[C@@](C(NCCC3=NC(C4=NC(C(O)=O)=CS4)=CS3)=O)([H])[C@@](O)([H])C)=O)([H])O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
122350-12?—
2350-11
from: 2350-11
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@]([C@@](C2=CNC=N2)([H])O)([H])C(N[C@@](C)([H])[C@@]([C@]([H])(C)C(N[C@@](C(NCCC3=NC(C4=NC(C(O)=O)=CS4)=CS3)=O)([H])[C@@](O)([H])C)=O)([H])O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
132350-13?—
2350-12
from: 2350-12
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@]([C@@](C2=CNC=N2)([H])O)([H])C(N[C@@](C)([H])[C@@]([C@]([H])(C)C(N[C@@](C(NCCC3=NC(C4=NC(C(O)=O)=CS4)=CS3)=O)([H])[C@@](O)([H])C)=O)([H])O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
142350-14?—
2350-13;terminal amine
from: 2350-13
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@]([C@@](C2=CNC=N2)([H])O)([H])C(N[C@@](C)([H])[C@@]([C@]([H])(C)C(N[C@@](C(NCCC3=NC(C4=NC(C(NCCC[S+](C)C)=O)=CS4)=CS3)=O)([H])[C@@](O)([H])C)=O)([H])O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
152350-15?—
2350-13;terminal amine
from: 2350-13
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@]([C@@](C2=CNC=N2)([H])O)([H])C(N[C@@](C)([H])[C@@]([C@]([H])(C)C(N[C@@](C(NCCC3=NC(C4=NC(C(NCCCCNC(N)=N)=O)=CS4)=CS3)=O)([H])[C@@](O)([H])C)=O)([H])O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
162350-16Start—
L-Gulose
O[C@H]1[C@H](O)[C@H](CO)O[C@@H](O)[C@H]1O—
172350-17Start—
3-O-Carbamoyl-D-mannose
O[C@@H]1[C@@H](O)[C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1—
182350(1)?—
2350-14;2350-16;2350-17
from: 2350-14, 2350-16, 2350-17
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@]([C@@](C2=CNC=N2)([H])C[C@H]3[C@@H](O[C@@H]4[C@@H](O)[C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O4)[C@@H](O)[C@H](O)[C@H](CO)O3)([H])C(N[C@@](C)([H])[C@@]([C@]([H])(C)C(N[C@@](C(NCCC5=NC(C6=NC(C(NCCC[S+](C)C)=O)=CS6)=CS5)=O)([H])[C@@](O)([H])C)=O)([H])O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
192350(2)?—
2350-15;2350-16;2350-17
from: 2350-15, 2350-16, 2350-17
O=C(N)C[C@](C1=NC(N)=C(C)C(C(N[C@]([C@@](C2=CNC=N2)([H])C[C@H]3[C@@H](O[C@@H]4[C@@H](O)[C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O4)[C@@H](O)[C@H](O)[C@H](CO)O3)([H])C(N[C@@](C)([H])[C@@]([C@]([H])(C)C(N[C@@](C(NCCC5=NC(C6=NC(C(NCCCCNC(N)=N)=O)=CS6)=CS5)=O)([H])[C@@](O)([H])C)=O)([H])O)=O)=O)=N1)([H])NC[C@]([H])(N)C(N)=O—
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