NPdia
← Back to Repository
BGC0002033PKS

spiramycin

Producing organism
Streptomyces ambofaciens ATCC 23877
Taxonomy
Bacteria›Actinomycetota›Actinomycetes›Kitasatosporales›Streptomycetaceae›Streptomyces
Biosynthetic class
PKS(Unknown)
Total steps
26
DOI
MIBiG entry
View on MIBiG ↗
Loading…
spiramycin
C43H74N2O14842.51 Da

Gene Cluster Map45 genes · 90,095 bp

KSKSKSKSKSKS20 kb90.1 kb visible · 90,095 bp total
Biosynthetic (core)
Biosynthetic (additional)
Transport
Other
Unknown
Domains
KSKetosynthase (KS)
ATAcyltransferase (AT)
DHDehydratase (DH)
EREnoylreductase (ER)
KRKetoreductase (KR)
ACPAcyl carrier protein (ACP)
ACPAcyl carrier (ACP)
ACPAcyl carrier (ACP)
TEThioesterase (TE)
DnDocking N-term
DcDocking C-term
FkFkbH-like
DHtDehydratase (DHt)
AmTAminotransferase I/II
NADNAD-binding

Pathway Graphsteps arranged in biosynthetic order

26 steps · 27 edges
Step 1Load
SAM23877_RS39245
+Malonyl-CoA
Step 10
start
+Glucose
Step 2M1
SAM23877_RS39245
+Malonyl-CoA
Step 11
SAM23877_RS26715
Step 3M2
SAM23877_RS39245
+Malonyl-CoA
Step 12
SAM23877_RS26710
Step 4M3
SAM23877_RS37920
+Malonyl-CoA
Step 13
SAM23877_RS26700
Step 18
SAM23877_RS41815
Step 21
SAM23877_RS26700
Step 5M4
SAM23877_RS26760
+Methylmalonyl-CoA
Inactive: KR
Step 14
SAM23877_RS26660
Step 19
SAM23877_RS26720
Step 22
SAM23877_RS26635
Step 6M5
SAM23877_RS26760
+Ethylmalonyl-CoA
Step 15
SAM23877_RS26675
Step 20
SAM23877_RS26690/SAM238…
Step 23
SAM23877_RS26630
Step 7M6
SAM23877_RS26765
+Methoxymalonyl-ACP
Step 16
SAM23877_RS26695
Step 24
SAM23877_RS26600
Step 8M7
SAM23877_RS26770
+Malonyl-CoA
Step 17
SAM23877_RS26690/SAM238…
Step 25
SAM23877_RS26640
Step 9TE
SAM23877_RS26770
Step 26
?
Starting stepIntermediateFinal product│ModuleLoadingTENo module│PKS stepNRPS stepTailoring/otherScroll to pan · Wheel to zoom

Biosynthetic Reactions26 steps

StepProduct IDEnzymeModuleSubstrateProduct SMILESNotes
12033-1SAM23877_RS39245Loading
Malonyl-CoA
OC(C)=O—
22033-2SAM23877_RS39245M1
2033-1;Malonyl-CoA
from: 2033-1
OC(C[C@H](O)C)=O—
32033-3SAM23877_RS39245M2
2033-2;Malonyl-CoA
from: 2033-2
OC(/C=C/C[C@H](O)C)=O—
42033-4SAM23877_RS37920M3
2033-3;Malonyl-CoA
from: 2033-3
OC(/C=C/C=C/C[C@H](O)C)=O—
52033-5SAM23877_RS26760M4
2033-4;Methylmalonyl-CoA
from: 2033-4
OC([C@@H](C)C(/C=C/C=C/C[C@H](O)C)=O)=O{'Inactive':['KR']}
62033-6SAM23877_RS26760M5
2033-5;Ethylmalonyl-CoA
from: 2033-5
OC([C@H](C[C@@H](C)C(/C=C/C=C/C[C@H](O)C)=O)CC)=O—
72033-7SAM23877_RS26765M6
2033-6;Methoxymalonyl-ACP
from: 2033-6
OC([C@H]([C@@H]([C@H](C[C@@H](C)C(/C=C/C=C/C[C@H](O)C)=O)CC)O)OC)=O—
82033-8SAM23877_RS26770M7
2033-7;Malonyl-CoA
from: 2033-7
O=C(C[C@H](O)[C@H]([C@@H]([C@H](C[C@@H](C)C(/C=C/C=C/C[C@H](O)C)=O)CC)O)OC)O—
92033-9SAM23877_RS26770TE
2033-8
from: 2033-8
O=C1C[C@@H](O)[C@@H]([C@@H](O)[C@@H](CC)C[C@@H](C)C(/C=C/C=C/C[C@@H](C)O1)=O)OC—
102033-10start—
Glucose
[H][C@@]1(O)[C@@]([H])(CO)O[C@]([H])(O[P-2])[C@]([H])(O)[C@@]1([H])O—
112033-11SAM23877_RS26715—
2033-10
from: 2033-10
[H][C@@]1(O)[C@@]([H])(CO)O[C@]([H])(O)[C@]([H])(O)[C@@]1([H])O—
122033-12SAM23877_RS26710—
2033-11
from: 2033-11
C[C@]1([H])C([C@@](O)([H])[C@@]([H])(O)[C@@]([H])(O)O1)=O—
132033-13SAM23877_RS26700—
2033-12
from: 2033-12
C[C@]1([H])C(C(C([H])([H])[C@@]([H])(O)O1)=O)=O—
142033-14SAM23877_RS26660—
2033-13
from: 2033-13
C[C@]1([H])C([C@@](O)([H])C([H])([H])[C@@]([H])(O)O1)=O—
152033-15SAM23877_RS26675—
2033-14
from: 2033-14
C[C@]1([H])C(C([H])([H])C([H])([H])[C@@]([H])(O)O1)=O—
162033-16SAM23877_RS26695—
2033-15
from: 2033-15
C[C@]1([H])C(N)C([H])([H])C([H])([H])[C@@]([H])(O)O1—
172033-17SAM23877_RS26690/SAM23877_RS26775—
2033-16
from: 2033-16
C[C@]1([H])C(N(C)C)C([H])([H])C([H])([H])[C@@]([H])(O)O1—
182033-18SAM23877_RS41815—
2033-12
from: 2033-12
C[C@]1([H])[C@@]([H])(O)C([C@@]([H])(O)[C@@]([H])(O)O1)=O—
192033-19SAM23877_RS26720—
2033-18
from: 2033-18
C[C@]1([H])[C@@]([H])(O)[C@@](N)([H])[C@@]([H])(O)[C@@]([H])(O)O1—
202033-20SAM23877_RS26690/SAM23877_RS26775—
2033-19
from: 2033-19
C[C@]1([H])[C@@]([H])(O)[C@]([H])(N(C)C)[C@@]([H])(O)[C@@]([H])(O)O1—
212033-21SAM23877_RS26700—
2033-12
from: 2033-12
C[C@]1([H])C(C(C([H])([H])[C@@]([H])(O)O1)=O)=O—
222033-22SAM23877_RS26635—
2033-21
from: 2033-21
C[C@]1([H])C([C@@]([H])(O)C([H])([H])[C@@]([H])(O)O1)=O—
232033-23SAM23877_RS26630—
2033-22
from: 2033-22
C[C@]1([H])C([C@@](O)(C)C([H])([H])[C@@]([H])(O)O1)=O—
242033-24SAM23877_RS26600—
2033-23
from: 2033-23
[H][C@]1(C)C([C@@](O)(C)C([H])([H])[C@@]([H])(O)O1)=O—
252033-25SAM23877_RS26640—
2033-24
from: 2033-24
[H][C@]1(C)[C@@](O)([H])[C@@](O)(C)C([H])([H])[C@@]([H])(O)O1—
262033?—
2033-9;2033-17;2033-20;2033-25
from: 2033-9, 2033-17, 2033-20, 2033-25
O=C1C[C@@H](O)[C@@H]([C@@H](OC2[C@H](O)[C@@H](N(C)C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@](C)(O)C3)[C@H](C)O2)[C@@H](CC)C[C@@H](C)[C@@H](O[C@H]4CC[C@H](N(C)C)[C@@H](C)O4)/C=C/C=C/C[C@@H](C)O1)OC—
Click any row to view the chemical structure · Click a module badge (M1, TE…) to view domain architecture