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BGC0000148PKS

A83543A;Spinosyn D

Producing organism: Saccharopolyspora spinosa
Taxonomy: Bacteria›Actinomycetota›Actinomycetes›Pseudonocardiales›Pseudonocardiaceae›Saccharopolyspora
Biosynthetic class: PKS (Type I)
Total steps: 27
DOI: 10.1016/S1074-5521(01)00029-1
MIBiG entry: View on MIBiG ↗

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C₄₁H₆₅NO₁₀731.46 Da

Gene Cluster Map23 genes · 80,161 bp

Biosynthetic (core)

Biosynthetic (additional)

Regulatory

Unknown

Domains

Ketosynthase (KS)

Acyltransferase (AT)

Dehydratase (DH)

Enoylreductase (ER)

Ketoreductase (KR)

Acyl carrier protein (ACP)

Acyl carrier (ACP)

Thioesterase (TE)

Docking N-term

Docking C-term

Methyltransferase

Beta-elim. lyase

Aminotransferase I/II

Pathway Graphsteps arranged in biosynthetic order

27 steps · 27 edges

Starting stepIntermediateFinal product│ModuleLoadingTENo module│PKS stepNRPS stepTailoring/otherScroll to pan · Wheel to zoom

Biosynthetic Reactions27 steps

Step	Product ID	Enzyme	Module	Substrate	Product SMILES	Notes
1	`148-1`	Start	—	Methylmalonyl-CoA	`O=C([R])CC`	—
2	`148-2`	spnA	Loading	148-1 from: 148-1	`O=C(O)CC`	—
3	`148-3`	spnA	M1	148-2;Malonyl-CoA from: 148-2	`OC(CC(O)=O)CC`	—
4	`148-4`	spnB	M2	148-3;Malonyl-CoA from: 148-3	`OC(CCCC(O)=O)CC`	—
5	`148-5`	spnC	M3	148-4;Methylmalonyl-CoA from: 148-4	`OC(CCCC(O)C(C)C(O)=O)CC`	—
6	`148-6`	spnC	M4	148-5;Malonyl-CoA from: 148-5	`OC(CCCC(O)C(C)C(O)CC(O)=O)CC`	—
7	`148-7`	spnD	M5	148-6;Malonyl-CoA from: 148-6	`OC(CCCC(O)C(C)C(O)C/C=C/C(O)=O)CC`	—
8	`148-8`	spnD	M6	148-7;Malonyl-CoA from: 148-7	`OC(CCCC(O)C(C)C(O)C/C=C/C(O)CC(O)=O)CC`	—
9	`148-9`	spnD	M7	148-8;Malonyl-CoA from: 148-8	`OC(CCCC(O)C(C)C(O)C/C=C/C(O)CC(O)CC(O)=O)CC`	—
10	`148-10`	spnE	M8	148-9;Malonyl-CoA from: 148-9	`OC(CCCC(O)C(C)C(O)C/C=C/C(O)CC(O)C/C=C/C(O)=O)CC`	—
11	`148-11`	spnE	M9	148-10;Malonyl-CoA from: 148-10	`OC(CCCC(O)C(C)C(O)C/C=C/C(O)CC(O)C/C=C/C=C/C(O)=O)CC`	—
12	`148-12`	spnE	M10	148-11;Malonyl-CoA from: 148-11	`OC(CCCC(O)C(C)C(O)C/C=C/C(O)CC(O)C/C=C/C=C/C=C/C(O)=O)CC`	—
13	`148-13`	spnE	TE	148-12 from: 148-12	`OC1C/C=C/C=C/C=C/C(OC(CC)CCCC(O)C(C)C(O)C/C=C/C(O)C1)=O`	—
14	`148-14`	spnF, spnJ, spnL, spnM	—	148-13 from: 148-13	`OC1CC2C=CC3C4C(C(C(C)C(O)CCCC(CC)OC(C4)=O)=O)=CC3C2C1`	—
15	`148-15`	Start	—	D-glucose-1-phosphate	`O[C@H]1[C@H](O)[C@@H](O)[C@@H](OP([O-])([O-])=O)O[C@@H]1CO`	—
16	`148-16`	?	—	148-15 from: 148-15	`O[C@H]1[C@H](O)[C@@H](O)[C@@H]([R])O[C@@H]1CO`	—
17	`148-17`	?	—	148-16 from: 148-16	`O[C@H]([C@@H](O)[C@@H]([R])O[C@@H]1C)C1=O`	—
18	`148-18`	?	—	148-17 from: 148-17	`O[C@@H]([C@@H]([R])O[C@H]1C)[C@H](O)C1=O`	—
19	`148-19`	?	—	148-18 from: 148-18	`O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1[R]`	—
20	`148-20`	spnO	—	148-17 from: 148-17	`C[C@H]1O[C@H]([R])CC(C1=O)=O`	—
21	`148-21`	spnN	—	148-20 from: 148-20	`C[C@H]1O[C@H]([R])C[C@@H](O)C1=O`	—
22	`148-22`	spnQ, spnR	—	148-21 from: 148-21	`C[C@H]1O[C@H]([R])CCC1N`	—
23	`148-23`	spnS	—	148-22 from: 148-22	`C[C@H]1O[C@H]([R])CCC1N(C)C`	—
24	`148-24`	spnG	—	148-14;148-19 from: 148-14, 148-19	`CCC(OC(CC1C2C3C4CC(O[C@@H]([C@H]5O)O[C@@H](C)C(O)[C@@H]5O)CC4C=C2)=O)CCCC(O)C(C)C(C1=C3)=O`	—
25	`148-25`	spnK, spnI, spnH	—	148-24 from: 148-24	`CCC(OC(CC1C2C3C4CC(O[C@@H]([C@H]5OC)O[C@@H](C)C(OC)[C@@H]5OC)CC4C=C2)=O)CCCC(O)C(C)C(C1=C3)=O`	—
26	`148(1)`	spnP	—	148-25;148-23 from: 148-25, 148-23	`CCC(OC(CC1C2C3C4CC(O[C@@H]([C@H]5OC)O[C@@H](C)C(OC)[C@@H]5OC)CC4C=C2)=O)CCCC(O[C@@H]6CCC(N(C)C)[C@@H](C)O6)C(C)C(C1=C3)=O`	—
27	`148(2)`	?	—	148(1)	`CCC(OC(CC1C2C3C4CC(O[C@@H]([C@H]5OC)O[C@@H](C)C(OC)[C@@H]5OC)CC4C(C)=C2)=O)CCCC(O[C@@H]6CCC(N(C)C)[C@@H](C)O6)C(C)C(C1=C3)=O`	—

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