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BGC0000136PKS

Rifamycin

Producing organism
Amycolatopsis mediterranei S699
Taxonomy
Bacteria›Actinomycetota›Actinomycetes›Pseudonocardiales›Pseudonocardiaceae›Amycolatopsis
Biosynthetic class
PKS (Unknown)
Total steps
31
DOI
MIBiG entry
View on MIBiG ↗
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rifamycin
C37H47NO12697.31 Da

Gene Cluster Map64 genes · 109,528 bp

20 kb109.5 kb visible · 109,528 bp total
Biosynthetic (core)
Biosynthetic (additional)
Regulatory
Transport
Other
Unknown
Domains
KSKetosynthase (KS)
ATAcyltransferase (AT)
DHDehydratase (DH)
KRKetoreductase (KR)
ACPAcyl carrier protein (ACP)
ACPAcyl carrier (ACP)
TEThioesterase (TE)
DnDocking N-term
DcDocking C-term
MTMethyltransferase
CALCoA-ligase (CAL)
AmTAminotransferase I/II
AmTAminotransferase III

Pathway Graphsteps arranged in biosynthetic order

31 steps · 30 edges
Step 1
Start
+UDP-glucose
Step 2
rifL
Step 3
rifK
Step 4
rifM
Step 5
rifN
Step 6
?
Step 7
(AAS07757.1, AAC01739.2…
Step 8
rifH
Step 9
rifG
Step 10
rifJ
Step 11
rifK
Step 12Load
rifA
Step 13M1
rifA
+Methylmalonyl-CoA
Inactive: DH
Step 14M2
rifA
+Malonyl-CoA
Step 15M3
rifA
+Methylmalonyl-CoA
Inactive: KR
Step 16
AAG52989.1
Step 17M4
rifB
+Methylmalonyl-CoA
Step 18M5
rifB
+Methylmalonyl-CoA
Inactive: DH
Step 19M6
rifB
+Methylmalonyl-CoA
Inactive: DH
Step 20M7
rifC
+Methylmalonyl-CoA
Inactive: DH
Step 21M8
rifD
+Methylmalonyl-CoA
Inactive: DH
Step 22M9
rifE
+Malonyl-CoA
Step 23M10
rifE
+Methylmalonyl-CoA
Step 24
rifF
Step 25
rifT, ?
Step 26
AAC01735.3, AAG52987.1?
Step 27
rifS?
Step 28
AAC01729.1
Step 29
AAC01728.1?
Step 30
AAG52990.1?
Step 31
AAC01738.1
Starting stepIntermediateFinal product│ModuleLoadingTENo module│PKS stepNRPS stepTailoring/otherScroll to pan · Wheel to zoom

Biosynthetic Reactions31 steps

StepProduct IDEnzymeModuleSubstrateProduct SMILESNotes
1136-1Start—
UDP-glucose
O[C@H]1[C@H](O)[C@@H](O)[C@@H]([R])O[C@@H]1CO—
2136-2rifL—
136-1
from: 136-1
O[C@H]1C([C@@H](O)[C@@H]([R])O[C@@H]1CO)=O—
3136-3rifK—
136-2
from: 136-2
O[C@H]1[C@H](N)[C@@H](O)[C@@H]([R])O[C@@H]1CO—
4136-4rifM—
136-3
from: 136-3
O[C@H]1[C@H](N)[C@@H](O)[C@@H](O)O[C@@H]1CO—
5136-5rifN—
136-4
from: 136-4
O[C@H]1[C@H](N)[C@@H](O)[C@@H](O)O[C@@H]1COP(O)(O)=O—
6136-6?—
136-5
from: 136-5
N[C@H]1[C@H](O)[C@H](O[C@@]1(CO)O)COP(O)(O)=O—
7136-7(AAS07757.1, AAC01739.2)?—
136-6
from: 136-6
[H][C@@](O)([C@](O)(COP(O)(O)=O)[H])C=N—
8136-8rifH—
136-7
from: 136-7
[H][C@@](O)([C@](O)(COP(O)(O)=O)[H])[C@@]([H])(C([H])(C(C(O)=O)=O)[H])N—
9136-9rifG—
136-8
from: 136-8
O=C1[C@@H](O)[C@H](N)C[C@](O)(C(O)=O)C1—
10136-10rifJ—
136-9
from: 136-9
O=C1[C@@H](O)[C@H](N)CC(C(O)=O)=C1—
11136-11rifK—
136-10
from: 136-10
OC1=CC(N)=CC(C(O)=O)=C1—
12136-12rifALoading
136-11
from: 136-11
OC1=CC(N)=CC(C(O)=O)=C1—
13136-13rifAM1
136-12;Methylmalonyl-CoA
from: 136-12
OC1=CC(N)=CC(C(O)C(C(O)=O)C)=C1{'Inactive': ['DH']}
14136-14rifAM2
136-13;Malonyl-CoA
from: 136-13
OC1=CC(N)=CC(C(O)C(C(CC(O)=O)=O)C)=C1—
15136-15rifAM3
136-14;Methylmalonyl-CoA
from: 136-14
OC1=CC(N)=CC(C(O)C(C(CC(C(C)C(O)=O)=O)=O)C)=C1{'Inactive': ['KR']}
16136-16AAG52989.1—
136-15
from: 136-15
O=C(C(C(O)=O)C)C1=C(O)C(C)C(O)C(C(C(N)=C2)=O)=C1C2=O—
17136-17rifBM4
136-16;Methylmalonyl-CoA
from: 136-16
O=C(/C(C)=C/C(C(O)=O)C)C1=C(O)C(C)C(O)C(C(C(N)=C2)=O)=C1C2=O—
18136-18rifBM5
136-17;Methylmalonyl-CoA
from: 136-17
O=C(/C(C)=C/C(C(O)C(C(O)=O)C)C)C1=C(O)C(C)C(O)C(C(C(N)=C2)=O)=C1C2=O{'Inactive': ['DH']}
19136-19rifBM6
136-18;Methylmalonyl-CoA
from: 136-18
O=C(/C(C)=C/C(C(O)C(C(O)C(C(O)=O)C)C)C)C1=C(O)C(C)C(O)C(C(C(N)=C2)=O)=C1C2=O{'Inactive': ['DH']}
20136-20rifCM7
136-19;Methylmalonyl-CoA
from: 136-19
O=C(/C(C)=C/C(C(O)C(C(O)C(C(O)C(C(O)=O)C)C)C)C)C1=C(O)C(C)C(O)C(C(C(N)=C2)=O)=C1C2=O{'Inactive': ['DH']}
21136-21rifDM8
136-20;Methylmalonyl-CoA
from: 136-20
O=C(/C(C)=C/C(C(O)C(C(O)C(C(O)C(C(O)C(C(O)=O)C)C)C)C)C)C1=C(O)C(C)C(O)C(C(C(N)=C2)=O)=C1C2=O{'Inactive': ['DH']}
22136-22rifEM9
136-21;Malonyl-CoA
from: 136-21
O=C(/C(C)=C/C(C(O)C(C(O)C(C(O)C(C(O)C(/C=C/C(O)=O)C)C)C)C)C)C1=C(O)C(C)C(O)C(C(C(N)=C2)=O)=C1C2=O—
23136-23rifEM10
136-22;Methylmalonyl-CoA
from: 136-22
O=C(/C(C)=C/C(C(O)C(C(O)C(C(O)C(C(O)C(/C=C/C=C(C(O)=O)\C)C)C)C)C)C)C1=C(O)C(C)C(O)C(C(C(N)=C2)=O)=C1C2=O—
24136-24rifF—
136-23
from: 136-23
CC1C(O)=C(C(/C(C)=C/C(C(C2C)O)C)=O)C(C(C=C(NC(/C(C)=C\C=C\C(C(O)C(C)C(O)C(C)C2O)C)=O)C3=O)=O)=C3C1O—
25136-25rifT, ?—
136-24
from: 136-24
CC1=C(O)C2=C(C(C=C(NC(/C(C)=C\C=C\C(C(O)C(C)C(O)C(C)C3O)C)=O)C2=O)=O)C(C(/C(C)=C/C(C(C3C)O)C)=O)=C1O—
26136-26AAC01735.3, AAG52987.1?—
136-25
from: 136-25
CC1=C(O)C2=C(C(C=C(NC(/C(C)=C\C=C\C(C(O)C(C)C(O)C(C)C3O)C)=O)C2=O)=O)C(C(/C(C)=C/C(C(C3C)O)CO)=O)=C1O—
27136-27rifS?—
136-26
from: 136-26
CC1=C(O)C2=C(C(C=C(NC(/C(C)=C\C=C\C(C(O)C(C)C(O)C(C)C3OC4=[R])C)=O)C2=O)=O)C(C(/C(C)=C/C4C(C3C)O)=O)=C1O—
28136-28AAC01729.1—
136-27
from: 136-27
O=C1C(O/C=C/C(O)C(C)C(O)C(C)C(O)C(C)C(O)C(C)/C=C/C=C2/C)(C)OC3=C1C(C(C=C4NC2=O)=O)=C(C(O)=C3C)C4=O—
29136-29AAC01728.1?—
136-28
from: 136-28
O=C1C(O/C=C/C(O)C(C)C(O)C(C)C(O)C(C)C(O)C(C)/C=C/C=C2/C)(C)OC3=C1C(C(O)=C4)=C(C(O)=C3C)C(O)=C4NC2=O—
30136-30AAG52990.1?—
136-29
from: 136-29
O=C1C(O/C=C/C(O)C(C)C(OC(C)=O)C(C)C(O)C(C)C(O)C(C)/C=C/C=C2/C)(C)OC3=C1C(C(O)=C4)=C(C(O)=C3C)C(O)=C4NC2=O—
31136AAC01738.1—
136-30
from: 136-30
O=C1C(O/C=C/C(OC)C(C)C(OC(C)=O)C(C)C(O)C(C)C(O)C(C)/C=C/C=C2/C)(C)OC3=C1C(C(O)=C4)=C(C(O)=C3C)C(O)=C4NC2=O—
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