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BGC0000028PKS

Bafilomycin B1

Producing organism
Streptomyces lohii
Taxonomy
Bacteria›Actinomycetota›Actinomycetes›Kitasatosporales›Streptomycetaceae›Streptomyces
Biosynthetic class
PKS (Type I)
Total steps
21
DOI
MIBiG entry
View on MIBiG ↗
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bafilomycin B1
C44H65NO13815.45 Da

Gene Cluster Map18 genes · 74,009 bp

AAKSKSKSKSKSKSKSKSKSKSKS10 kb74.0 kb visible · 74,009 bp total
Biosynthetic (core)
Biosynthetic (additional)
Regulatory
Unknown
Domains
KSKetosynthase (KS)
ATAcyltransferase (AT)
DHDehydratase (DH)
EREnoylreductase (ER)
KRKetoreductase (KR)
ACPAcyl carrier protein (ACP)
ACPAcyl carrier (ACP)
AAdenylation (A)
ACPAcyl carrier (ACP)
TEThioesterase (TE)
DnDocking N-term
DcDocking C-term
FkFkbH-like
AmTAminotransferase I/II

Pathway Graphsteps arranged in biosynthetic order

21 steps · 20 edges
Step 1Load
ADC79616.1
+Isobutyric acid
Step 14
Start
+Fumarate
Step 16
Start
+Glycine
Step 17
Start
+Succinyl-CoA
Step 2M1
ADC79616.1
+Methylmalonyl-CoA
Step 18
ADC79615.1
Step 3M2
ADC79616.1
+Malonyl-CoA
Step 19
ADC79613.1
Step 4M3
ADC79616.1
+Methylmalonyl-CoA
Step 20
ADC79615.1
Step 5M4
ADC79617.1
+Methylmalonyl-CoA
Step 6M5
ADC79617.1
+Methoxymalonyl-ACP
Step 7M6
ADC79617.1
+Malonyl-CoA
Step 8M7
ADC79618.1
+Methylmalonyl-CoA
Step 9M8
ADC79618.1
+Methylmalonyl-CoA
Step 10M9
ADC79619.1
+Methylmalonyl-CoA
Step 11M10
ADC79619.1
+Methylmalonyl-CoA
Step 12M11
ADC79620.1
+Methoxymalonyl-ACP
Step 13TE
ADC79620.1
Step 15
?
Step 21
ADC79614.1
Starting stepIntermediateFinal product│ModuleLoadingTENo module│PKS stepNRPS stepTailoring/otherScroll to pan · Wheel to zoom

Biosynthetic Reactions21 steps

StepProduct IDEnzymeModuleSubstrateProduct SMILESNotes
128-1ADC79616.1Loading
Isobutyric acid
CC(C(O)=O)C—
228-2ADC79616.1M1
28-1;Methylmalonyl-CoA
from: 28-1
CC([C@@H](O)[C@H](C(O)=O)C)—
328-3ADC79616.1M2
28-2;Malonyl-CoA
from: 28-2
CC([C@@H](O)[C@H]([C@H](O)CC(O)=O)C)C—
428-4ADC79616.1M3
28-3;Methylmalonyl-CoA
from: 28-3
CC([C@@H](O)[C@H]([C@H](O)CC([C@H](C(O)=O)C)=O)C)C—
528-5ADC79617.1M4
28-4;Methylmalonyl-CoA
from: 28-4
CC([C@@H](O)[C@H]([C@H](O)CC([C@H]([C@H](O)[C@@H](C(O)=O)C)C)=O)C)C—
628-6ADC79617.1M5
28-5;Methoxymalonyl-ACP
from: 28-5
CC([C@@H](O)[C@H]([C@H](O)CC([C@H]([C@H](O)[C@@H]([C@@H](O)C(C(O)=O)OC)C)C)=O)C)C—
728-7ADC79617.1M6
28-6;Malonyl-CoA
from: 28-6
CC([C@@H](O)[C@H]([C@H](O)CC([C@H]([C@H](O)[C@@H]([C@@H](O)C(/C=C/C(O)=O)OC)C)C)=O)C)C—
828-8ADC79618.1M7
28-7;Methylmalonyl-CoA
from: 28-7
CC([C@@H](O)[C@H]([C@H](O)CC([C@H]([C@H](O)[C@@H]([C@@H](O)C(/C=C/C=C(C(O)=O)\C)OC)C)C)=O)C)C—
928-9ADC79618.1M8
28-8;Methylmalonyl-CoA
from: 28-8
CC([C@@H](O)[C@H]([C@H](O)CC([C@H]([C@H](O)[C@@H]([C@@H](O)C(/C=C/C=C(C[C@@H](C(O)=O)C)\C)OC)C)C)=O)C)C—
1028-10ADC79619.1M9
28-9;Methylmalonyl-CoA
from: 28-9
CC([C@@H](O)[C@H]([C@H](O)CC([C@H]([C@H](O)[C@@H]([C@@H](O)C(/C=C/C=C(C[C@@H]([C@H](O)[C@@H](C(O)=O)C)C)\C)OC)C)C)=O)C)C—
1128-11ADC79619.1M10
28-10;Methylmalonyl-CoA
from: 28-10
CC([C@@H](O)[C@H]([C@H](O)CC([C@H]([C@H](O)[C@@H]([C@@H](O)C(/C=C/C=C(C[C@@H]([C@H](O)[C@@H](/C=C(C(O)=O)\C)C)C)\C)OC)C)C)=O)C)C—
1228-12ADC79620.1M11
28-11;Methoxymalonyl-ACP
from: 28-11
CC([C@@H](O)[C@H]([C@H](O)CC([C@H]([C@H](O)[C@@H]([C@@H](O)C(/C=C/C=C(C[C@@H]([C@H](O)[C@@H](/C=C(/C=C(C(O)=O)\OC)C)C)C)\C)OC)C)C)=O)C)C—
1328-13ADC79620.1TE
28-12
from: 28-12
O[C@@]1([C@H]([C@@H]([C@@H]([C@H]([C@H](/C=C/C=C(C[C@@H]([C@@H]([C@@H](/C=C(/C=C2\OC)C)C)O)C)\C)OC)OC2=O)C)O)C)O[C@H](C(C)C)[C@@H](C)[C@H](O)C1—
1428-14Start—
Fumarate
OC(/C=C/C(O)=O)=O—
1528-15?—
28-13;28-14
from: 28-13, 28-14
O=C(O[C@@H]1C[C@@]([C@H]([C@@H]([C@@H]([C@H]([C@H](/C=C/C=C(C[C@@H]([C@@H]([C@@H](/C=C(/C=C2\OC)C)C)O)C)\C)OC)OC2=O)C)O)C)(O)O[C@H](C(C)C)[C@H]1C)/C=C/C(O)=O—
1628-16Start—
Glycine
NCC(O)=O—
1728-17Start—
Succinyl-CoA
[R]CC(CCC(O)=O)=O—
1828-18ADC79615.1—
28-16;28-17
from: 28-16, 28-17
NCC(CCC(O)=O)=O—
1928-19ADC79613.1—
28-18
from: 28-18
NCC(CCC([R])=O)=O—
2028-20ADC79615.1—
28-19
from: 28-19
O=C1CC(O)=CC1N—
2128ADC79614.1—
28-15;28-20
from: 28-15, 28-20
O=C(O[C@@H]1C[C@@]([C@H]([C@@H]([C@@H]([C@H]([C@H](/C=C/C=C(C[C@@H]([C@@H]([C@@H](/C=C(/C=C2\OC)C)C)O)C)\C)OC)OC2=O)C)O)C)(O)O[C@H](C(C)C)[C@H]1C)/C=C/C(NC3=C(O)CCC3=O)=O—
Click any row to view the chemical structure · Click a module badge (M1, TE…) to view domain architecture